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Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Inventor-of-fluorescence-Ploemopak-in-running-for-Nobel-Prize-website-on-his-early-technology-tp7580208p7580218.html
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Oh dear, nostalgia. Fred Rost was a wonderful microscopist and a great professor (Anatomy, University of NSW) but a bit off-putting at first encounter since he always dressed (totally unconvincingly) as a woman. But yes, he certainly knew the history of fluorescence microscopy.
Guy
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of George McNamara
Sent: Sunday, 21 April 2013 2:58 AM
To:
[hidden email]
Subject: Re: Inventor of fluorescence Ploemopak in running for Nobel Prize + website on his early technology
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On 4/20/2013 11:08 AM, John Oreopoulos wrote:
F.W.D. Rost published three books with extensive (occasionally tedious) history of fluorescence microscopy.
Page 187 to 188 of Fluorescence Microscopy volume 2:
http://books.google.com/books?id=-eJsstG_ClcC&printsec=frontcover&dq=Fluorescence+microscopy.+2&hl=en&sa=X&ei=ZcVyUerSOoy89QTak4GABQ&ved=0CDwQ6AEwAA#v=onepage&q=Fluorescence%20microscopy.%202&f=false
1992 Fluorescence Microscopy - volume 1
http://books.google.com/books/about/Fluorescence_microscopy_1.html?id=RU_6doL1q0IC
Fluorescence microscopy is used for studying the distribution of
substances which are present in very small amounts. The high
sensitivity of the method makes it ideal for studying the
distribution of substances in living cells. Its techniques are used
mainly in biology and medicine, but are also valuable in coal
petrology and elsewhere. The best-known application is in
immunofluorescence. This magnificent new work provides comprehensive
coverage of all aspects of fluorescence microscopy. It covers
instrumentation, applications to a wide variety of fields, and the
history of the technique. There is a chapter on quantitative
techniques, including scanning: this aspect is dealt with in more
detail in a companion volume, Quantitative Fluorescence Microscopy.
Volume 1 deals with instrumentation and techniques for fluorescence
microscopy, and includes the chapter on quantitation and scanning.
Volume 2 deals with the applications of fluorescence microscopy in
many fields. It includes information on fluorochromes and on
autofluorescence. An invaluable appendix provides an alphabetical
list of fluorochromes, giving information concerning chemical
structure, fluorescence properties, applications and suitable filter
combinations. These two important volumes will be of use to all
fluorescence microscopists and will be an invaluable reference tool
for those graduate students and research workers in biology,
medicine and earth science who need to make use of these techniques.
1991 Quantitative fluorescence microscopy
http://books.google.com/books?id=G3cC7WQMArYC&source=gbs_similarbooks Quantitative fluorescence microscopy is concerned with making
measurements from fluorescent specimens in a fluorescence
microscope, by measuring fluorescence emission from a defined area
or areas of a specimen. This technique is most commonly used to
determine the amount of some specific substance, such as DNA, in
some particular area of a cell. But it has many other uses; for
example, it can be used to identify certain substances in the cell
by examining their fluorescence characteristics. This book is a
complete guide to this technique for all biologists. It describes
the principles and applications of quantitative fluorescence
microscopy and also gives much practical information about the
instrumentation required. There is also a discussion of the exciting
developments in confocal fluorescence microscopy which allows the
three dimensional distribution of particular substances to be
determined. Everyone presently using this technique, or wishing to
start using it will need to read this book.
Enjoy,
George
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
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>
> Mark,
>
> Your last posting peaked my curiosity, so I decided to look a bit into this. The best I could come up with was a document by Barry Masters on the history of fluorescence microscopy:
>
>
http://www.google.ca/url?sa=t&rct=j&q=&esrc=s&source=web&cd=11&ved=0CE> MQFjAAOAo&url=http%3A%2F%2Fwww.fen.bilkent.edu.tr%2F~physics%2Fnews%2F
> masters%2FELS_Hist_Fl_Micro.pdf&ei=4rlyUe2hLtGp4APHr4GwCg&usg=AFQjCNEW
> 1u-TzRGu5wml8GZ26qGUN9iW3A&sig2=HnzzQ9CvfTkEvfJWVcfCeg&bvm=bv.45512109
> ,d.dmg&cad=rja
>
> Both Brumberg and Ploem are mentioned in the context of some very important developments of epi-fluorescence microscopy. By chance, does anyone have a copy of the papers cited involving these authors? (Brumberg 1959, and Ploem 1967).
>
> John Oreopoulos
> Staff Scientist
> Spectral Applied Research
> Richmond Hill, Ontario
> Canada
> www.spectral.ca
>
>
> On 2013-04-19, at 5:44 PM, Mark Cannell wrote:
>
>
>> *****
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>>
>> I hope the pioneering work in 1948 of Evengenii Mikhailovich Brumberg is mentioned/considered in this context.
>>
>> Cheers
>>
>>
[hidden email]
>>
>