FRET in Arabidopsis- Clover/Ruby

Posted by Christian-103 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-for-confocal-training-tp7580015p7580243.html

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In the next few weeks, I have a user who will need FRET analysis performed in Arabidopsis intact leaves.  He is using the new Clover/Ruby constructs which express very well in the plant material. 

The problem quite bluntly, is that I have not had any FRET training in 10 years except a brief webinar, which was advertised on this list-serv.  In any case, I work on a Nikon A1 with the FRET package in NIS-Elements, but also have a spectral detector.  If anyone would like to offer tips here or off list, I'd be very appreciative.  Any tutorials would be very beneficial as well. 

Thank you!

Christian