Re: Inventor of fluorescence Ploemopak in running for Nobel Prize + website on his early technology

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Inventor-of-fluorescence-Ploemopak-in-running-for-Nobel-Prize-website-on-his-early-technology-tp7580208p7580260.html

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On 4/25/2013 4:19 AM, Guy Cox wrote:

> I do think that the pioneers of immuno-fluorescence also deserve a Nobel!

Now THERE is a prize that would've been worth presenting!  Sadly, Albert
Coons, the father of immunofluorescence, died in 1978, but there are
other worthies who contributed to the widespread usefulness of the
technique.

Having used a fluorescence microscope with a sub-stage darkfield
condenser, I can say that the system has at least one advantage: the
brightness of the image was (partly) a function of the NA of that
condenser.  If you had dim labeling and want to get a low-mag image of
it, diascopic illumination was a good way to go.

Martin Wessendorf


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