Colocalization on Z-stack images

Posted by Marjan Gharagozloo on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Colocalization-on-Z-stack-images-tp7580319.html

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Dear All,

I'd like to study colocalization of Draq5 and Cy3 in Nucleus.
Actually, this topic is totally new to me and I've read many articles and
manuals to find a way for quantifying colocalization. However, I don't
know how I can analyze colocalization in my Zstack images. I know by
changing focal plane, I'll get different results and quantities. It's
kind of you if send me some information to do this analysis.

Many thanks and best regards:
Marjan

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Best regards:

Marjan Gharagozloo (PhD)
Postdoctoral Fellow
University of Waterloo
School of Pharmacy, PHR3002
10 Victoria St S, N2G 2B2
Kitchener, ON, Canada