single molecule bleaching correction

Posted by Jeff Spector on
URL: http://confocal-microscopy-list.275.s1.nabble.com/single-molecule-bleaching-correction-tp7580328.html

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Greetings,
 this may be somewhat out of place in this listserv but I know a lot of good
microscopists are on this list.
 I'm using a single molecule assay to try and measure the binding kinetics of an
enzyme. Essentially, I can see them land on my substrate and then disappear. I
would like to measure the on and off rates but am unsure as to how to include
the effects of photobleaching. I have seen a lot of papers where people "correct"
for this but don't explain how. Could someone on this list point me towards some
literature that might help me out?
thanks...