Posted by
John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/single-molecule-bleaching-correction-tp7580328p7580329.html
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Jeff,
Take a look at these articles. Might be some help here:
Coffman, V.C. and J.Q. Wu, Counting protein molecules using quantitative fluorescence microscopy. Trends in Biochemical Sciences, 2012. 37(11): p. 499-506.
McGuire, H., et al., Automating single subunit counting of membrane proteins in mammalian cells. Journal of Biological Chemistry, 2012. 287(43): p. 35912-35921.
John Oreopoulos
On 2013-05-09, at 10:07 PM, Jeff Spector wrote:
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> Greetings,
> this may be somewhat out of place in this listserv but I know a lot of good
> microscopists are on this list.
> I'm using a single molecule assay to try and measure the binding kinetics of an
> enzyme. Essentially, I can see them land on my substrate and then disappear. I
> would like to measure the on and off rates but am unsure as to how to include
> the effects of photobleaching. I have seen a lot of papers where people "correct"
> for this but don't explain how. Could someone on this list point me towards some
> literature that might help me out?
> thanks...