Posted by
Chris Tully on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tetracycline-to-bone-augmentation-tp7580337p7580338.html
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Javier,
I am NOT a Pathologist, but for the last three years have been providing
demonstrations of digital pathology equipment to pathologists across the
world. I have left that position and opened a consulting company, Image
Incyte, LLC.
I only saw bone samples a few times in my multiple demos. What I do recall
though is: Errors in animal treatments have been identified by
characteristic tetracycline rings on bones - meaning that you do not have
to specifically stain for tetracycline, it shows up on it's own. I believe
that the drug is actually incorporated whole in the bone and shows up with
distinctive fluorescence.
I found this note:
http://www.histosearch.com/histonet/Dec02/TetracyclinelabelingofbonA.htmlon the Wikipedia page for tetracycline:
http://en.wikipedia.org/wiki/Tetracycline#cite_note-mayton-15I hope that helps you get started on your search.
Chris Tully
Microscopy and Image Analysis Expert
Image Incyte, LLC
www.ImageIncyte.com
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240-475-9753 (c)
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On Wed, May 15, 2013 at 10:16 AM, Javier Adur <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear list,
> We want to evaluate the bone augmentation in rabbit tibia using
> Tetracycline or similar.
> Does anyone know a protocol of inmunohistochemistry o reference literature
> about it.
> Thanks in advance.
> Javier
>
>
>
>
>
>
>
>
> Dr. Javier F. Adur
>
> Docente/Investigador
> - Facultad de Ingeniería - UNER - Argentina
>
> Laboratorio de
> Microscopia Aplicada a Estudios Celulares y Moleculares
>
> Cátedra Radiaciones
> No Ionizantes
>
>