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mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tetracycline-to-bone-augmentation-tp7580337p7580341.html
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In the early 1990s I imaged what sounds like this for a renal MD. He had bone biopsies from patients given tetracycline as an indicator of bone growth which was a diagnostic tool for success/failure or kidney transplant. The question was whether the confocal could avoid the prep of the standard technique of getting a neat cross section. But we could not image depth of tetracycline ring accurately by looking at the surface and trying to do a Z series. And for imaging cross sections, we found the confocal was no improvement over the then clinical practice. Back then we were using a 60X NA 1.4 Nikon fixed tube length objective; maybe newer microscopes could do better.
Regards,
Michael C.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Jeremy Adler
Sent: Thursday, May 16, 2013 3:28 AM
To:
[hidden email]
Subject: Re: Tetracycline to bone augmentation
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tetracycline fluoresces
Quoting Javier Adur <
[hidden email]>:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear list,
> We want to evaluate the bone augmentation in rabbit tibia using
> Tetracycline or similar.
> Does anyone know a protocol of inmunohistochemistry o reference
> literature about it.
> Thanks in advance.
> Javier
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> Dr. Javier F. Adur
>
> Docente/Investigador
> - Facultad de Ingeniería - UNER - Argentina
>
> Laboratorio de
> Microscopia Aplicada a Estudios Celulares y Moleculares
>
> Cátedra Radiaciones
> No Ionizantes
>
>
Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden
0046 (0)18 471 4607