Posted by
Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Tetracycline-to-bone-augmentation-tp7580337p7580342.html
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Without a doubt you still need sectioning to measure bone deposition by tetracycline fluorescence. Bone is only 10-30% tissue and at least 70% hydroxyapatite, a mineral that does not transmit light. This property has given my current research group, which very much would like to see bone cells in their natural environment, more than a little bit of grief.
cheers,
TF
Timothy Feinstein, PhD
Visiting Research Associate
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA 15261
On May 16, 2013, at 11:43 AM, Cammer, Michael wrote:
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> In the early 1990s I imaged what sounds like this for a renal MD. He had bone biopsies from patients given tetracycline as an indicator of bone growth which was a diagnostic tool for success/failure or kidney transplant. The question was whether the confocal could avoid the prep of the standard technique of getting a neat cross section. But we could not image depth of tetracycline ring accurately by looking at the surface and trying to do a Z series. And for imaging cross sections, we found the confocal was no improvement over the then clinical practice. Back then we were using a 60X NA 1.4 Nikon fixed tube length objective; maybe newer microscopes could do better.
> Regards,
> Michael C.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Jeremy Adler
> Sent: Thursday, May 16, 2013 3:28 AM
> To:
[hidden email]
> Subject: Re: Tetracycline to bone augmentation
>
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> tetracycline fluoresces
>
>
> Quoting Javier Adur <
[hidden email]>:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> *****
>>
>> Dear list,
>> We want to evaluate the bone augmentation in rabbit tibia using
>> Tetracycline or similar.
>> Does anyone know a protocol of inmunohistochemistry o reference
>> literature about it.
>> Thanks in advance.
>> Javier
>>
>>
>>
>>
>>
>>
>>
>>
>> Dr. Javier F. Adur
>>
>> Docente/Investigador
>> - Facultad de Ingeniería - UNER - Argentina
>>
>> Laboratorio de
>> Microscopia Aplicada a Estudios Celulares y Moleculares
>>
>> Cátedra Radiaciones
>> No Ionizantes
>>
>>
>
>
>
> Jeremy Adler
> IGP
> Rudbeckslaboratoriet
> Daghammersköljdsväg 20
> 751 85 Uppsala
> Sweden
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> 0046 (0)18 471 4607