Re: Beads for rapid protein release during imaging

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Beads-for-rapid-protein-release-during-imaging-tp7580390p7580391.html

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Robert Langer, MIT is the scientist most associated with slow or fast
release matrices. See

http://web.mit.edu/langerlab/publications/2001.html
(may need other years)

He also cofounded    
http://www.modernatx.com/about?gclid=CM7_g6_7vrcCFUdk7AoddjIANg     -
you might be able to work with them on samples.

George

On 5/30/2013 12:44 PM, Eric Olson wrote:

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> Dear List,
>
> I am looking for beads that I can load with recombinant proteins, wash in
> saline and then inject into a brain explant. The idea is to provide a
> localized source of the protein that releases over the course of a few hours
> (not days) - while I am imaging.
>
> When I have injected the protein by itself, the protein becomes widely
> distributed very rapidly (minutes) which is not good for these experiments.
>
> Ideally, the beads would not be larger than 10 micrometers in diameter and
> could be fluorescent.
>
> Does anyone have any experience with this sort of application?
>
> Thanks,
>
> Eric
>
>    

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George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054