Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580403.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi Nicola,
LaVision Biotec sells the "Ultramicroscope". Former colleagues at UMiami
told me this instrument is giving them excellent images of YFP
expressing neurons (in a subset of cells) of the entire mouse brains in
3 minutes at 1 um 'resolution' (they may mean pixel size). They have
improved on the Erturk ... Dodt et al recipe:
1: Ertürk A, Becker K, Jährling N, Mauch CP, Hojer CD, Egen JG, Hellal F, Bradke
F, Sheng M, Dodt HU. Three-dimensional imaging of solvent-cleared organs using
3DISCO. Nat Protoc. 2012 Nov;7(11):1983-95. doi: 10.1038/nprot.2012.119. Epub
2012 Oct 11. PubMed PMID: 23060243.
2: Ertürk A, Mauch CP, Hellal F, Förstner F, Keck T, Becker K, Jährling N,
Steffens H, Richter M, Hübener M, Kramer E, Kirchhoff F, Dodt HU, Bradke F.
Three-dimensional imaging of the unsectioned adult spinal cord to assess axon
regeneration and glial responses after injury. Nat Med. 2011 Dec 25;18(1):166-71.
doi: 10.1038/nm.2600. PubMed PMID: 22198277.
which they find works better than Scale. See:
Luo X, Salgueiro Y, Beckerman SR, Lemmon VP, Tsoulfas P, Park KK.
Three-dimensional evaluation of retinal ganglion cell axon regeneration
and pathfinding in whole mouse tissue after injury. Exp Neurol. 2013 Mar
16. doi:pii: S0014-4886(13)00084-8. 10.1016/j.expneurol.2013.03.001.
[Epub ahead of print] PubMed PMID: 23510761.
As for focusing only on current commercial systems: big mistake.
See
http://www.focusonmicroscopy.org/2013/index.html for lots of
activity in this field, and especially
Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA
lenses and clever image processing)
http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdfGeorge
On 6/4/2013 4:13 AM, Nicola Green wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi
> This is not strictly confocal microscopy, but I hope you might be able to
> help still.
> I am interested in using the light sheet fluorescence/single plane
> illumination microscopy technique for imaging live 3D tissue engineered
> constructs. I know that Zeiss sell the Lightsheet Z1 system that does this.
> Has anyone had any experience with using this and can comment on it or do
> you know of any other similar commercially available systems?
> I know that many people report building their own systems but I am not
> thinking to go down that route at the moment.
> Thanks for your help
> Regards
> Nicola
>
>
--
George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054