Posted by
Johannes Schindelin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580404.html
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Hi George & Nicola,
On Tue, 4 Jun 2013, George McNamara wrote:
> On 6/4/2013 4:13 AM, Nicola Green wrote:
> >
> > I am interested in using the light sheet fluorescence/single plane
> > illumination microscopy technique for imaging live 3D tissue engineered
> > constructs. I know that Zeiss sell the Lightsheet Z1 system that does this.
> > Has anyone had any experience with using this and can comment on it or do
> > you know of any other similar commercially available systems?
> >
> > I know that many people report building their own systems but I am not
> > thinking to go down that route at the moment.
>
> [...]
>
> As for focusing only on current commercial systems: big mistake.
>
> See
http://www.focusonmicroscopy.org/2013/index.html for lots of
> activity in this field, and especially
>
> Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA lenses
> and clever image processing)
>
>
http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdfInteresting. A (not all too) quick web search found no details about the
setup, just beautiful images.
I would like to point to a project I am personally involved in (together
with a couple of other list regulars) and whose focus is primarily to make
light-sheet microscopy accessible:
http://openspim.org/. It contains a
detailed parts list and instructions how to build it even if you are not
an optics expert, along with fully Open Source control software.
The key to the OpenSPIM is that it is an accessible platform, i.e. it can
be extended and enhanced very easily.
For example, I imagine that once information about Wu and Shroff's dual
view setup becomes available, someone will come up with minimal
modifications to the OpenSPIM setup to replicate the same results, and for
maximal impact that someone could extend
http://openspim.org/ (which is a
Wiki) to describe those modifications so that other people can easily
rebuild that setup, too.
Ciao,
Johannes