Posted by
Johannes Schindelin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580405.html
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Hi,
On Tue, 4 Jun 2013, Johannes Schindelin wrote:
> On Tue, 4 Jun 2013, George McNamara wrote:
>
> > On 6/4/2013 4:13 AM, Nicola Green wrote:
> > >
> > > I am interested in using the light sheet fluorescence/single plane
> > > illumination microscopy technique for imaging live 3D tissue
> > > engineered constructs. I know that Zeiss sell the Lightsheet Z1
> > > system that does this. Has anyone had any experience with using
> > > this and can comment on it or do you know of any other similar
> > > commercially available systems?
> > >
> > > I know that many people report building their own systems but I am
> > > not thinking to go down that route at the moment.
> >
> > [...]
> >
> > As for focusing only on current commercial systems: big mistake.
> >
> > See
http://www.focusonmicroscopy.org/2013/index.html for lots of
> > activity in this field, and especially
> >
> > Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA lenses
> > and clever image processing)
> >
> >
http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdf>
> Interesting. A (not all too) quick web search found no details about the
> setup, just beautiful images.
There is actually a paper which I found linked from some related NIH page
describing the system:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462167/?report=reader#!po=65.9091In their Data Processing section, they have a link to the software hosted
on Google Code which actually contains a Wiki where they describe how to
rebuild their setup:
http://code.google.com/p/msim/wiki/Building_your_own_MSIMI added a link to
http://openspim.org/Links.
Note that in the Deconvolution section of above-linked paper, it is stated
that they use the Parallel Iterative Deconvolution plugin for Image, but
of course they mean ImageJ.
Ciao,
Johannes