Re: Light sheet fluorescence microscopy

Posted by Raghu Parthasarathy on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580407.html

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   One commercial light sheet implementation that hasn't been mentioned:  ASI sells the "inverted SPIM" setup that they & Hari Shroff et al. developed (www.pnas.org/cgi/doi/10.1073/pnas.1108494108):  http://www.asiimaging.com/products/light-sheet-microscopy/inverted-selective-plane-illumination-microscopy-ispim/ .

   I haven't used any of the commercial setups; it's fun and not particularly hard to build one's own light sheet microscope.  The OpenSPIM wiki noted in another reply seems quite good, by the way.

best wishes,

Raghu


 
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Raghuveer Parthasarathy
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Group: http://physics.uoregon.edu/~raghu/
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Associate Professor
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1274 University of Oregon
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________________________________
 From: Nicola Green <[hidden email]>
To: [hidden email]
Sent: Tuesday, June 4, 2013 2:13 AM
Subject: Light sheet fluorescence microscopy
 

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Hi
This is not strictly confocal microscopy, but I hope you might be able to
help still.
I am interested in using the light sheet fluorescence/single plane
illumination microscopy technique for imaging live 3D tissue engineered
constructs. I know that Zeiss sell the Lightsheet Z1 system that does this.
Has anyone had any experience with using this and can comment on it or do
you know of any other similar commercially available systems?
I know that many people report building their own systems but I am not
thinking to go down that route at the moment.
Thanks for your help
Regards
Nicola