Re: Light sheet fluorescence microscopy

Posted by Nuno Moreno on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580408.html

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Hi,

For micromanager and open source hardware fans please have a look at this DSLM/SPIM/OPT configuration

https://sites.google.com/site/openspinmicroscopy/

Coming on nature methods (july issue) as correspondence.

This system uses arduino boards and a custom build micromanager interface for dealing with sample rotation, galvo-speed, timelapse, etc, etc. Using this methodology you can setup a "spin" microscope for less than 50k euros even with topnotch camera and objective.

Best,
Nuno Moreno, PhD
Instituto Gulbenkian de Ciência
http://lnkd.in/6VXcrM





On Jun 4, 2013, at 4:16 PM, Douglas Richardson wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Hello Nicola,
>
> Zeiss is currently the only commercial manufacturer of a Lightsheet
> system.  We just installed the first system in North America in our
> facility, not sure how many have already been installed in Europe.  We're
> very impressed with the system.
>
> Feel free to contact me off-list for more details.
>
> -Doug
>
> http://labs.mcb.harvard.edu/hcbi/
>
>
> On Tue, Jun 4, 2013 at 5:13 AM, Nicola Green <[hidden email]>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Hi
>> This is not strictly confocal microscopy, but I hope you might be able to
>> help still.
>> I am interested in using the light sheet fluorescence/single plane
>> illumination microscopy technique for imaging live 3D tissue engineered
>> constructs. I know that Zeiss sell the Lightsheet Z1 system that does this.
>> Has anyone had any experience with using this and can comment on it or do
>> you know of any other similar commercially available systems?
>> I know that many people report building their own systems but I am not
>> thinking to go down that route at the moment.
>> Thanks for your help
>> Regards
>> Nicola
>>