Re: Light sheet fluorescence microscopy

Posted by Douglas Richardson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580409.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

Just wanted to apologize for my earlier post.  When I stated the Z1 was the
only commercial implementation of a lightsheet microscope I was just
  referring to the big 4 (Zeiss/Leica/Nikon/Olympus). As indicated by other
posts, other commercial implementations exist.

Sorry for the confusion,

-Doug


On Tue, Jun 4, 2013 at 12:29 PM, Nuno Moreno <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> For micromanager and open source hardware fans please have a look at this
> DSLM/SPIM/OPT configuration
>
> https://sites.google.com/site/openspinmicroscopy/
>
> Coming on nature methods (july issue) as correspondence.
>
> This system uses arduino boards and a custom build micromanager interface
> for dealing with sample rotation, galvo-speed, timelapse, etc, etc. Using
> this methodology you can setup a "spin" microscope for less than 50k euros
> even with topnotch camera and objective.
>
> Best,
> Nuno Moreno, PhD
> Instituto Gulbenkian de Ciência
> http://lnkd.in/6VXcrM
>
>
>
>
>
> On Jun 4, 2013, at 4:16 PM, Douglas Richardson wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hello Nicola,
> >
> > Zeiss is currently the only commercial manufacturer of a Lightsheet
> > system.  We just installed the first system in North America in our
> > facility, not sure how many have already been installed in Europe.  We're
> > very impressed with the system.
> >
> > Feel free to contact me off-list for more details.
> >
> > -Doug
> >
> > http://labs.mcb.harvard.edu/hcbi/
> >
> >
> > On Tue, Jun 4, 2013 at 5:13 AM, Nicola Green <[hidden email]
> >wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Hi
> >> This is not strictly confocal microscopy, but I hope you might be able
> to
> >> help still.
> >> I am interested in using the light sheet fluorescence/single plane
> >> illumination microscopy technique for imaging live 3D tissue engineered
> >> constructs. I know that Zeiss sell the Lightsheet Z1 system that does
> this.
> >> Has anyone had any experience with using this and can comment on it or
> do
> >> you know of any other similar commercially available systems?
> >> I know that many people report building their own systems but I am not
> >> thinking to go down that route at the moment.
> >> Thanks for your help
> >> Regards
> >> Nicola
> >>
>