autofluorescence in liver tissue
Posted by
Cromey, Douglas W - (dcromey) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/autofluorescence-in-liver-tissue-tp7580412.html
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I am working with a lab looking at IF stained sections of formalin-fixed, paraffin embedded pieces of mouse or rat liver. If there was any actual staining in their first try on our confocal, it was largely washed out by the lovely and strong autofluorescence. Because their animal experiment takes 8 weeks, it's not trivial (or inexpensive) to get new tissue, so they have been using existing blocks. No one in their lab had the foresight to put up snap-frozen tissues for cryosectioning. I have them looking at this document:
http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf but I'm wondering if liver is always a problem with autofluorescence, or is there maybe something in this lab's sample prep protocol that's making it worse. Any ideas?
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
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