Posted by
Mark A. Sanders on
URL: http://confocal-microscopy-list.275.s1.nabble.com/autofluorescence-in-liver-tissue-tp7580412p7580418.html
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Hi Doug,
The liver autofluorescence may be due to lipofuscin. Try treating with Sudan Black. Sudan Black binds lipophilic compartments and, being black, absorbs the excitation energy and quenches lipofuscin.
Here is a paper:
Schnell SA, Staines WA, Wessendorf MW. Reduction of lipofuscin-like
autofluorescence in fluorescently labeled tissue. J Histochem Cytochem.
1999 47(6):719-30.
Cheers,
Mark
****************************************************
Mark A. Sanders University of Minnesota
Program Director Twin Cities Campus
University Imaging Centers
St. Paul office ph: 612-624-3454
Mpls office ph: 612-626-3645
fax: 612-624-1799
www.uic.umn.edu
On Jun 4, 2013, at 12:23 PM, "Cromey, Douglas W - (dcromey)" <
[hidden email]> wrote:
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> I am working with a lab looking at IF stained sections of formalin-fixed, paraffin embedded pieces of mouse or rat liver. If there was any actual staining in their first try on our confocal, it was largely washed out by the lovely and strong autofluorescence. Because their animal experiment takes 8 weeks, it's not trivial (or inexpensive) to get new tissue, so they have been using existing blocks. No one in their lab had the foresight to put up snap-frozen tissues for cryosectioning. I have them looking at this document:
http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf but I'm wondering if liver is always a problem with autofluorescence, or is there maybe something in this lab's sample prep protocol that's making it worse. Any ideas?
>
> Doug
>
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular & Molecular Medicine, University of Arizona
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