Re: Light sheet fluorescence microscopy

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580422.html

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Hi Johannes,

Hari Shoff and his lab have a very good track record on making their
plans and code available - see

https://code.google.com/p/msim/

for details of their 2012 MSIM paper (PubMed 22581372 ).

Hopefully the "instant SIM" (another FOM 2013 abstract) and dual view
SPIM will be published soon (Nature Methods?) and the computational
explanation will be explained and code - and program - made available.
You could always email or call Hari to find out what they did for dual
view SPIM to make it isotropic.

George



On 6/4/2013 9:25 AM, Johannes Schindelin wrote:

> Hi George&  Nicola,
>
> On Tue, 4 Jun 2013, George McNamara wrote:
>
>    
>> On 6/4/2013 4:13 AM, Nicola Green wrote:
>>      
>>> I am interested in using the light sheet fluorescence/single plane
>>> illumination microscopy technique for imaging live 3D tissue engineered
>>> constructs. I know that Zeiss sell the Lightsheet Z1 system that does this.
>>> Has anyone had any experience with using this and can comment on it or do
>>> you know of any other similar commercially available systems?
>>>
>>> I know that many people report building their own systems but I am not
>>> thinking to go down that route at the moment.
>>>        
>> [...]
>>
>> As for focusing only on current commercial systems: big mistake.
>>
>> See http://www.focusonmicroscopy.org/2013/index.html   for lots of
>> activity in this field, and especially
>>
>> Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA lenses
>> and clever image processing)
>>
>> http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdf
>>      
> Interesting. A (not all too) quick web search found no details about the
> setup, just beautiful images.
>
> I would like to point to a project I am personally involved in (together
> with a couple of other list regulars) and whose focus is primarily to make
> light-sheet microscopy accessible: http://openspim.org/. It contains a
> detailed parts list and instructions how to build it even if you are not
> an optics expert, along with fully Open Source control software.
>
> The key to the OpenSPIM is that it is an accessible platform, i.e. it can
> be extended and enhanced very easily.
>
> For example, I imagine that once information about Wu and Shroff's dual
> view setup becomes available, someone will come up with minimal
> modifications to the OpenSPIM setup to replicate the same results, and for
> maximal impact that someone could extend http://openspim.org/ (which is a
> Wiki) to describe those modifications so that other people can easily
> rebuild that setup, too.
>
> Ciao,
> Johannes
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054