http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580426.html
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> Hi Johannes,
>
> Hari Shoff and his lab have a very good track record on making their plans
> and code available - see
>
>
https://code.google.com/p/msim/>
> for details of their 2012 MSIM paper (PubMed 22581372 ).
>
> Hopefully the "instant SIM" (another FOM 2013 abstract) and dual view SPIM
> will be published soon (Nature Methods?) and the computational explanation
> will be explained and code - and program - made available. You could always
> email or call Hari to find out what they did for dual view SPIM to make it
> isotropic.
>
> George
>
>
>
> On 6/4/2013 9:25 AM, Johannes Schindelin wrote:
>>
>> Hi George& Nicola,
>>
>> On Tue, 4 Jun 2013, George McNamara wrote:
>>
>>
>>>
>>> On 6/4/2013 4:13 AM, Nicola Green wrote:
>>>
>>>>
>>>> I am interested in using the light sheet fluorescence/single plane
>>>> illumination microscopy technique for imaging live 3D tissue engineered
>>>> constructs. I know that Zeiss sell the Lightsheet Z1 system that does
>>>> this.
>>>> Has anyone had any experience with using this and can comment on it or
>>>> do
>>>> you know of any other similar commercially available systems?
>>>>
>>>> I know that many people report building their own systems but I am not
>>>> thinking to go down that route at the moment.
>>>>
>>>
>>> [...]
>>>
>>> As for focusing only on current commercial systems: big mistake.
>>>
>>> See
http://www.focusonmicroscopy.org/2013/index.html for lots of
>>> activity in this field, and especially
>>>
>>> Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA
>>> lenses
>>> and clever image processing)
>>>
>>>
http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdf>>>
>>
>> Interesting. A (not all too) quick web search found no details about the
>> setup, just beautiful images.
>>
>> I would like to point to a project I am personally involved in (together
>> with a couple of other list regulars) and whose focus is primarily to make
>> light-sheet microscopy accessible:
http://openspim.org/. It contains a
>> detailed parts list and instructions how to build it even if you are not
>> an optics expert, along with fully Open Source control software.
>>
>> The key to the OpenSPIM is that it is an accessible platform, i.e. it can
>> be extended and enhanced very easily.
>>
>> For example, I imagine that once information about Wu and Shroff's dual
>> view setup becomes available, someone will come up with minimal
>> modifications to the OpenSPIM setup to replicate the same results, and for
>> maximal impact that someone could extend
http://openspim.org/ (which is a
>> Wiki) to describe those modifications so that other people can easily
>> rebuild that setup, too.
>>
>> Ciao,
>> Johannes
>>
>>
>
>
>
> --
>
>
>
> George McNamara, Ph.D.
> Single Cells Analyst
> L.J.N. Cooper Lab
> University of Texas M.D. Anderson Cancer Center
> Houston, TX 77054