Re: How to show something inside the cell?

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-show-something-inside-the-cell-tp7580451p7580452.html

Hi Yi - I think your solution is good. Fluorophores on the surface can also be selectively quenched, depending on the fluorophore - FITC by lowering pH, red fluorophores or those emitting in the 600s by adding several mg/ml of Acid Blue 9.

Mike Model


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Subject: How to show something inside the cell?

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Dear listers,

One of our user is investigating the transportation of fluorescence labeled DNA oligo in cells. He would like to prove the DNA oligo has been transported inside the cells but not on the surface of cells by using the light microscopy. We suggested him to clearly label the membrane of cells and use the Z-stack images of confocal to look through the cells.
I am wondering if there is any other better solution?

Any suggestion on good membrane dye is appreciated as well!

Thank you,

Yi