Invitrogen/Life Tech Neurotrace Gel protocol

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Invitrogen-Life-Tech-Neurotrace-Gel-protocol-tp7580454.html

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Hello all, I recently got my hands on a Neurotrace Gel kit from Life Tech
(Invitrogen) and want to use it in a non-standard way.  The kit I have is
the DiO, DiL, and DiD combo.  I want to use one or two of these dyes to
label cultured neurons in a dish.  The problem is that the Gels are
normally meant for tissue, and you just sort-of smear the gell directly
onto a tissue slice or the like.  The cells we have are just cultured on
the bottom of a dish in some PBS.  Any thoughts as to how one might get the
Water-resistant Gel containing Di into the cells?  I was thinking of trying
an emulsion into the PBS but I'm not sure that would work.

Thoughts?

Craig