Posted by
Kilgore, Jason-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-show-something-inside-the-cell-tp7580451p7580461.html
**vendor reply**
A common method would be to use a quencher in the cell media, to quench any surface fluorescence.
A traditional method would be to use trypan blue. However, this is very broad-range -- a bad thing if you wish to quench only a selected dye in a multiplex situation
Perhaps a better method would be to use one of the BackDrop fluorescence quenching reagents, which can be targeted to blue, green or red. And they come in handy dropper-bottle formulations. Link:
https://products.invitrogen.com/ivgn/product/R37603?ICID=search-productAny dye that is internalized in live cells, with either of these options, will be fluorescent.
Cheers,
Jason
Jason A. Kilgore
Technical Application Scientist
Molecular Probes Labeling and Detection Technologies
Cells Systems Division
T 1 800 955 6288 then option 4, then option 6, or 541 335 0353 • F 541 335 0238
29851 Willow Creek Rd • Eugene • OR • 97402-9132 • United States
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Dear listers,
One of our user is investigating the transportation of fluorescence labeled
DNA oligo in cells. He would like to prove the DNA oligo has been
transported inside the cells but not on the surface of cells by using the
light microscopy. We suggested him to clearly label the membrane of cells
and use the Z-stack images of confocal to look through the cells.
I am wondering if there is any other better solution?
Any suggestion on good membrane dye is appreciated as well!
Thank you,
Yi