Posted by
Peter Gabriel Pitrone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Light-sheet-fluorescence-microscopy-tp7580400p7580469.html
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Hello Listers,
I thought I would bring to your attention two correspondences in Nature Methods on light sheet
microscopy...
http://www.nature.com/nmeth/journal/vaop/ncurrent/index.htmlHave a great work week!!
Pete
--
Peter Gabriel Pitrone - TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden
"If a straight line fit is required, obtain only two data points." - Anon.
On Wed, June 5, 2013 5:08 am, phil laissue wrote:
<|> *****
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<|>
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<|>
<|> light sheet microscopy was a main theme at this year's FOM, great to
<|> see it take centre stage, and worth checking out several of the FOM
<|> abstracts for this.
<|> _____________________________________
<|> Philippe Laissue, PhD, Bioimaging Manager
<|> School of Biological Sciences, Room 4.17
<|> University of Essex, Colchester CO4 3SQ, UK
<|> (0044) 01206 872246 / (0044) 07842 676 456
<|>
[hidden email]
<|> privatewww.essex.ac.uk/~plaissue
<|>
<|>
<|> On Wed, Jun 5, 2013 at 1:19 AM, George McNamara
<|> <
[hidden email]> wrote:
<|>> *****
<|>> To join, leave or search the confocal microscopy listserv, go to:
<|>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy<|>> *****
<|>>
<|>> Hi Johannes,
<|>>
<|>> Hari Shoff and his lab have a very good track record on making their plans
<|>> and code available - see
<|>>
<|>>
https://code.google.com/p/msim/<|>>
<|>> for details of their 2012 MSIM paper (PubMed 22581372 ).
<|>>
<|>> Hopefully the "instant SIM" (another FOM 2013 abstract) and dual view SPIM
<|>> will be published soon (Nature Methods?) and the computational explanation
<|>> will be explained and code - and program - made available. You could always
<|>> email or call Hari to find out what they did for dual view SPIM to make it
<|>> isotropic.
<|>>
<|>> George
<|>>
<|>>
<|>>
<|>> On 6/4/2013 9:25 AM, Johannes Schindelin wrote:
<|>>>
<|>>> Hi George& Nicola,
<|>>>
<|>>> On Tue, 4 Jun 2013, George McNamara wrote:
<|>>>
<|>>>
<|>>>>
<|>>>> On 6/4/2013 4:13 AM, Nicola Green wrote:
<|>>>>
<|>>>>>
<|>>>>> I am interested in using the light sheet fluorescence/single plane
<|>>>>> illumination microscopy technique for imaging live 3D tissue engineered
<|>>>>> constructs. I know that Zeiss sell the Lightsheet Z1 system that does
<|>>>>> this.
<|>>>>> Has anyone had any experience with using this and can comment on it or
<|>>>>> do
<|>>>>> you know of any other similar commercially available systems?
<|>>>>>
<|>>>>> I know that many people report building their own systems but I am not
<|>>>>> thinking to go down that route at the moment.
<|>>>>>
<|>>>>
<|>>>> [...]
<|>>>>
<|>>>> As for focusing only on current commercial systems: big mistake.
<|>>>>
<|>>>> See
http://www.focusonmicroscopy.org/2013/index.html for lots of
<|>>>> activity in this field, and especially
<|>>>>
<|>>>> Wu and Shroff dual view isotropic 330 nm resolution (with 20x/0.8 NA
<|>>>> lenses
<|>>>> and clever image processing)
<|>>>>
<|>>>>
http://www.focusonmicroscopy.org/2013/PDF/159_Shroff.pdf<|>>>>
<|>>>
<|>>> Interesting. A (not all too) quick web search found no details about the
<|>>> setup, just beautiful images.
<|>>>
<|>>> I would like to point to a project I am personally involved in (together
<|>>> with a couple of other list regulars) and whose focus is primarily to make
<|>>> light-sheet microscopy accessible:
http://openspim.org/. It contains a
<|>>> detailed parts list and instructions how to build it even if you are not
<|>>> an optics expert, along with fully Open Source control software.
<|>>>
<|>>> The key to the OpenSPIM is that it is an accessible platform, i.e. it can
<|>>> be extended and enhanced very easily.
<|>>>
<|>>> For example, I imagine that once information about Wu and Shroff's dual
<|>>> view setup becomes available, someone will come up with minimal
<|>>> modifications to the OpenSPIM setup to replicate the same results, and for
<|>>> maximal impact that someone could extend
http://openspim.org/ (which is a
<|>>> Wiki) to describe those modifications so that other people can easily
<|>>> rebuild that setup, too.
<|>>>
<|>>> Ciao,
<|>>> Johannes
<|>>>
<|>>>
<|>>
<|>>
<|>>
<|>> --
<|>>
<|>>
<|>>
<|>> George McNamara, Ph.D.
<|>> Single Cells Analyst
<|>> L.J.N. Cooper Lab
<|>> University of Texas M.D. Anderson Cancer Center
<|>> Houston, TX 77054
<|>