http://confocal-microscopy-list.275.s1.nabble.com/32-bit-monitor-tp7580506p7580510.html
Yes, each channel still has 8 bits (8 x 4 = 32). AFAIK you cannot buy a commercial monitor that displays more than 8 bits in any one channel.
Q for Andreas: Do you use any visualization software on the scope computer other than the proprietary package from Zeiss? If I had to guess I'd suggest that Zen 'cleans up' images a little on-screen in a way that does not carry over when you open the same image in a non-allied software package. I noticed that Elements for Nikon does this, for example smoothing the edges of pixels at > 100% zoom and de-noising a little. Open your images in ImageJ on that same computer and see whether they look substantially better than ImageJ somewhere else. My bet is that you just need to apply a little contrast, noise filtering and (DANGER Will Robinson) gamma in third party software to reproduce what you see in Zen.
Dept. of Pharmacology & Chemical Biology
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>
> '32 bit' could be rgb+alpha… I don't think Windoze goes higher than 8 bits per channel?
> The resolution might relate to the number of pixels your display has. If you have a image of 2048x2048 you need an unusual monitor to display it at full resolution.
>
> Cheers
>
>
> On 13/06/2013, at 4:52 PM, Keith Morris <
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Hi Andreas
>>
>> Assuming that your PC or MAC monitors are set to 32-bit true-colour under
>> Display, Adjust Resolution, Advanced Settings (windows 7), the problem is
>> probably the PC monitor isn't set up correctly (colour, brightness,
>> contrast). I'd get an x-rite i1 Display Pro professional display calibration
>> device to calibrate your office monitors (xritephoto.com) - it costs about
>> £150 and can generally calibrate all your screens automatically assuming
>> they are fairly modern (the sensor unit rests on the monitor and adjusts it
>> all correctly for you). Then in theory all your PC monitors will be
>> calibrated to display colours correctly, so the image should look similar on
>> any of them, subject to the basic quality of the monitor. I'd also
>> investigate the likes of a £400 2560x1440 pixel 27" Dell U2713HM IPS screen
>> monitor for your main office Windows PC which can display 1024x1024 confocal
>> images natively at 1 pixel per 1 pixel. Use Zen/LSM Image Browser to view
>> your Zeiss raw lsm confocal files to ensure the software isn't down-sampling
>> the image in some way. I doubt the graphics card is the problem, assuming
>> it's fairly modern graphics card/GPU and it's been setup correctly.
>>
>> Regards
>>
>> Keith
>>
>>
http://xritephoto.com/ph_product_overview.aspx?id=1454&catid=109&action=over>> view
>>
>> -----------------------------------------------------------
>> Dr Keith J Morris
>> Cellular Imaging Core,
>> The Wellcome Trust Centre for Human Genetics,
>> Roosevelt Drive,
>> Oxford,
>> OX3 7BN,
>> United Kingdom.
>>
>> Tel: +44 ( 0 ) 1865 287568
>> Email:
[hidden email]
>> Webpage: www.well.ox.ac.uk/microscopy-facilities
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:
[hidden email]] On
>> Behalf Of Andreas Bruckbauer
>> Sent: 13 June 2013 15:52
>> To:
[hidden email]
>> Subject: 32 bit monitor
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>> We noticed that our confocal images look amazing (fine details well
>> resolved) as long as they are viewed with the monitor belonging to the
>> microscope, but as soon as we open them on our PCs or Macs they look just
>> normal (fine detail missing).
>> The monitor at the Zeiss mic is a HP ZR30W a pretty expensive model. It
>> seems to support 32 bit colours which would give 12 bit or 4096 grey steps
>> for each of the primary colours. Not sure if this is the secret behind the
>> good looking images but has anyone experience using such monitors to display
>> images? I would think that the graphic card, operating system and image
>> analysis software have to be capable of doing so, which programs would do
>> this?
>>
>> Did i miss an important development in computer hardware or is there another
>> reason for the amazing looking images? Any suggestions welcome.
>
> Mark B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology & Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
>
[hidden email]