Sample Prep: Frozen sections not 'sticking' to slides

Posted by Ellen T. Arena on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sample-Prep-Frozen-sections-not-sticking-to-slides-tp7580551.html

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Hello all -

I am actually having a sample-prep issue - I am hoping someone can help...  

I am wanting to mount 'thick' frozen sections (~30um) of tissue on
SuperFrost slides for staining/imaging.  I am finding the tissues come 'off'
quite easily during the staining/washing procedure.  I have used the typical
methods with OCT-removal (with PBS), fixation with PFA, etc.  I am not quite
sure if any 'trick' exists for these thicker sections that I might be
missing...  ???

Please let me know if you have any advice.

Many thanks.

Ellen


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Ellen T. Arena, PhD
Pasteur Foundation Postdoctoral Fellow
Unité de Pathogénie Microbienne Moléculaire
INSERM U786
Institut Pasteur
28 rue du Dr Roux
F - 75724 PARIS Cédex 15
France
Tel: [phone]
Fax: [phone]
[hidden email]
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