http://confocal-microscopy-list.275.s1.nabble.com/Sample-Prep-Frozen-sections-not-sticking-to-slides-tp7580551p7580557.html
-Use Super Frost Plus slides which has a charge to it and it will prevent the sections from falling off during IHC processing.
For us, the super frost plus slides worked well. I found these tricks on the IHC World website (
). Good luck.
> Date: Tue, 25 Jun 2013 03:47:40 -0500
> From:
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> Subject: Sample Prep: Frozen sections not 'sticking' to slides
> To:
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> Hello all -
>
> I am actually having a sample-prep issue - I am hoping someone can help...
>
> I am wanting to mount 'thick' frozen sections (~30um) of tissue on
> SuperFrost slides for staining/imaging. I am finding the tissues come 'off'
> quite easily during the staining/washing procedure. I have used the typical
> methods with OCT-removal (with PBS), fixation with PFA, etc. I am not quite
> sure if any 'trick' exists for these thicker sections that I might be
> missing... ???
>
> Please let me know if you have any advice.
>
> Many thanks.
>
> Ellen
>
>
> -----------------------------------------------------------------------------
> Ellen T. Arena, PhD
> Pasteur Foundation Postdoctoral Fellow
> Unité de Pathogénie Microbienne Moléculaire
> INSERM U786
> Institut Pasteur
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