http://confocal-microscopy-list.275.s1.nabble.com/Sample-Prep-Frozen-sections-not-sticking-to-slides-tp7580551p7580564.html
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> I agree with Judy. Additionally, if you use gelatin or poly-L-lysine
> slides, you can do an aldehyde fixative after mounting the section, which
> will crosslink in place the tissue with they lysines.
>
> Jason
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
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> On Behalf Of Drazba, Ph.D., Judy
> Sent: Tuesday, June 25, 2013 7:40 AM
> To:
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> Subject: Re: Sample Prep: Frozen sections not 'sticking' to slides
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> Hi Ellen,
>
> We do this all the time but find that frozen sections, especially thick
> ones, only stick to Superfrost Plus slides. Regular Superfrost slides don't
> work. Whatever they do to make them + (plus) - makes all the difference.
> You can always tell which slides your using because the Plus slides have
> little + on the corners.
>
> Hope this helps.
> Judy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
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> On Behalf Of Ellen T. Arena
> Sent: Tuesday, June 25, 2013 4:48 AM
> To:
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> Subject: Sample Prep: Frozen sections not 'sticking' to slides
>
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> Hello all -
>
> I am actually having a sample-prep issue - I am hoping someone can help...
>
> I am wanting to mount 'thick' frozen sections (~30um) of tissue on
> SuperFrost slides for staining/imaging. I am finding the tissues come 'off'
> quite easily during the staining/washing procedure. I have used the
> typical methods with OCT-removal (with PBS), fixation with PFA, etc. I am
> not quite sure if any 'trick' exists for these thicker sections that I
> might be missing... ???
>
> Please let me know if you have any advice.
>
> Many thanks.
>
> Ellen
>
>
>
> -----------------------------------------------------------------------------
> Ellen T. Arena, PhD
> Pasteur Foundation Postdoctoral Fellow
> Unité de Pathogénie Microbienne Moléculaire INSERM U786 Institut Pasteur
> 28 rue du Dr Roux
> F - 75724 PARIS Cédex 15
> France
> Tel: (33-0) 1 40 61 37 71
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