slowing down or immobilizing (somewhat) purified fluorescent protein

Posted by Jen Jackson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/slowing-down-or-immobilizing-somewhat-purified-fluorescent-protein-tp7580579.html

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Hello all,


I'm looking for a method to 'slow down', but not completely immobilize- a
low concentration of YFP particles to help characterize a TIRF system.
Ideally, we could perhaps later use this "YFP gel" to calibrate fluorescence
intensity of single YFP molecules in biological membranes, but for now I'm
looking for a decent way to have some sort of reduction in particle speed,
as compared to solution, without compromising the spectral properties (etc.)
of the protein.  Any tips, tricks?


Many thanks,

Jen