Re: slowing down or immobilizing (somewhat) purified fluorescent protein

Posted by Michelle Peckham on
URL: http://confocal-microscopy-list.275.s1.nabble.com/slowing-down-or-immobilizing-somewhat-purified-fluorescent-protein-tp7580579p7580580.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Attach the YFP to the glass surface, using an antibody

On 28/06/2013 20:08, "Jen Jackson" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hello all,
>
>
>I'm looking for a method to 'slow down', but not completely immobilize- a
>low concentration of YFP particles to help characterize a TIRF system.
>Ideally, we could perhaps later use this "YFP gel" to calibrate
>fluorescence
>intensity of single YFP molecules in biological membranes, but for now I'm
>looking for a decent way to have some sort of reduction in particle speed,
>as compared to solution, without compromising the spectral properties
>(etc.)
>of the protein.  Any tips, tricks?
>
>
>Many thanks,
>
>Jen