Posted by
Smith, Benjamin E. on
Jun 29, 2013; 12:08am
URL: http://confocal-microscopy-list.275.s1.nabble.com/slowing-down-or-immobilizing-somewhat-purified-fluorescent-protein-tp7580579p7580588.html
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You may also be able to use a sieving matrix such as polyacrylamide or
low melting point agarose to spatially constrain the molecules.
Depending on the pore size they still should rotate freely, but their
lateral diffusion should be greatly slowed.
If you find something that works, I'd love to know, as I also have a PI
who needs to slow down the Brownian motion of quantum dots while keeping
them in an aqueous solution.
-Ben Smith
Benjamin E. Smith, Ph.D.
Samuel Roberts Noble Microscopy Laboratory
Research Scientist II
University of Oklahoma
Norman, OK 73019
E-mail:
[hidden email]
Voice 405-325-4391
FAX 405-325-7619
http://www.microscopy.ou.edu/On 6/28/2013 3:06 PM, Jen Jackson wrote:
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> Thanks for your response. I would prefer the molecules for the have even
> more mobility so to test temporal resolution. But antibodies are a great
> start- can you recommend any protocol for attaching antibodies to a
> coverslip (just use poly-d-lysine, or?).