Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/slowing-down-or-immobilizing-somewhat-purified-fluorescent-protein-tp7580579p7580594.html
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Maybe I am missing something here, but how about just adding glycerol to
the solution? Other options might be a low concentration of
low-melting-point agarose or a gel used for ultrasonic examinations.
Steffen
On 28.06.2013 21:08, Jen Jackson wrote:
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> Hello all,
>
>
> I'm looking for a method to 'slow down', but not completely immobilize- a
> low concentration of YFP particles to help characterize a TIRF system.
> Ideally, we could perhaps later use this "YFP gel" to calibrate fluorescence
> intensity of single YFP molecules in biological membranes, but for now I'm
> looking for a decent way to have some sort of reduction in particle speed,
> as compared to solution, without compromising the spectral properties (etc.)
> of the protein. Any tips, tricks?
>
>
> Many thanks,
>
> Jen
>
--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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