Re: slowing down or immobilizing (somewhat) purified fluorescent protein

Posted by Jen Jackson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/slowing-down-or-immobilizing-somewhat-purified-fluorescent-protein-tp7580579p7580604.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Thanks all for your input!  

In regards to something like glycerol- for my purposes, the high refractive
index will cause problems with TIR.  So my question is now clearer: which
gels would act as the best imaging medium for TIRF?  Cygel was mentioned to
have a refractive index of 1.36, so could be a good candidate.  However it's
also somewhat expensive, so if anyone has used agarose, or other materials
suggested in this thread- successfully with TIRF microscopy- I would be
interested to hear about it.

Thanks!

Jen