Re: Colocalization on Z-stack images

Posted by Müller,Tobias on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Colocalization-on-Z-stack-images-tp7580319p7580672.html

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Helo Marjan,

You might find the "jacop" plugin for Fiji useful. I works in 3D, is quite user-friendly and contains a link to a nice coloc-paper giving you the necessary background to operate it.

http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:jacop_2.0:just_another_colocalization_plugin:start

Best,
Tobias


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Marjan Gharagozloo
Sent: Wednesday, May 08, 2013 5:45 PM
To: [hidden email]
Subject: Colocalization on Z-stack images

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Dear All,

I'd like to study colocalization of Draq5 and Cy3 in Nucleus.
Actually, this topic is totally new to me and I've read many articles and manuals to find a way for quantifying colocalization. However, I don't know how I can analyze colocalization in my Zstack images. I know by changing focal plane, I'll get different results and quantities. It's kind of you if send me some information to do this analysis.

Many thanks and best regards:
Marjan

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Best regards:

Marjan Gharagozloo (PhD)
Postdoctoral Fellow
University of Waterloo
School of Pharmacy, PHR3002
10 Victoria St S, N2G 2B2
Kitchener, ON, Canada