Posted by
rjpalmer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorescent-staining-of-living-S-Pombe-tp7580863p7580866.html
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Laurent -
Calcofluor or other cellulose-binders are typical, caveat being I
don't work with yeasts.. A membrane stain called FUN has also been
used. See papers with Ghannoum in the author line.
Rob
On Aug 15, 2013, at 5:46 AM, Gelman, Laurent wrote:
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> Hello everybody,
>
>
> We would need to stain living S. Pombe cells imaged on a
> fluorescence microscope to segment thereafter during image analysis
> automatically the cells.
> Staining cell wall and/or membrane would be perfect. Has someone any
> specific experience with yeast and/or Pombe?
>
> Note that we need an organic dye and cannot express any recombinant
> protein (we are screening a yeast library).
>
> Thanks for your advices!
>
> Best regards,
>
>
> Laurent.
>
>
>
> _____________________________________________
> Laurent Gelman, PhD
> Head of Facility for Advanced Imaging and Microscopy
> (Light Microscopy)
>
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Robert J. Palmer Jr., Ph.D.
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