Posted by
Theresa Swayne on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorescent-staining-of-living-S-Pombe-tp7580863p7580868.html
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Dear Laurent,
Fluorescent Concanavalin A and Calcofluor both work well for S. cerevisiae.
Calcofluor is the cell wall dye used in the FUN-1 (Live/Dead) yeast kit.
The Ohya and Morishita labs used FITC-ConA to generate the Saccharomyces cerevisiae Morphological Database,
http://scmd.gi.k.u-tokyo.ac.jp/datamine/.
If you are doing long-term imaging, ConA might be better as you could choose wavelengths that would avoid UV exposure.
Hope this helps!
Theresa
On Aug 15, 2013, at 5:46 AM, "Gelman, Laurent" <
[hidden email]> wrote:
>
> We would need to stain living S. Pombe cells imaged on a fluorescence microscope to segment thereafter during image analysis automatically the cells.
> Staining cell wall and/or membrane would be perfect. Has someone any specific experience with yeast and/or Pombe?
>
> Note that we need an organic dye and cannot express any recombinant protein (we are screening a yeast library).
>
>
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Theresa C. Swayne, Ph.D.
Manager, Confocal and Specialized Microscopy Shared Resource
Herbert Irving Comprehensive Cancer Center, Columbia University
1130 Saint Nicholas Ave, 222A
New York, NY 10032
212-851-4613
[hidden email]
http://hiccc.columbia.edu/research/sharedresources/confocal