Re: SIM redux

Posted by Dan Metcalf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/SIM-redux-tp7580938p7580951.html

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Hi Michael,

I suspect in this case, as Ian Dobbie has suggested, that this is a reconstruction
artefact. However, I have seen a labelling artefact that was resolved by SIM and
STORM using instruments built at the National Physical Laboratory but otherwise
not resolvable by conventional imaging. In this case, a large secretory granule
type structure. The immunolabelling was against the content protein but the
antibodies weren't able to penetrate the very centre, which created a hollow core
type structure as the dyes ended up outside.

Regards,

Dan Metcalf

Nikon Instruments UK