Posted by
mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/manual-stitching-problem-tp7581006p7581007.html
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In image properties in Imarus you should be able to swap Z and T.
-mc
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Yevgeniy Romin
Sent: Monday, September 23, 2013 11:04 AM
To:
[hidden email]
Subject: manual stitching problem
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Dear List
I am currently having the following problem. I ran a timelapse using the Leica SP8 software, it was a 3x3 tilescan z-stack with 100 timeframes. There was a lot of cell movement in the timelapse, and the automatic stitching in the Leica software did not do a very good job of stitching the images together. All I need is to stitch together the maximum projection of the z-stack by manually aligning them. I have tried cropping the projection into it's respective 9 parts, both in Imaris and in LAS, since it is impossible to get the single tile files from the original lif file in the Leica software. Then I have tried stitching these in XUV stitch, but the software sees the timepoints in both *.lif and *.ims files that I cropped as z-planes instead of timepoints, so I am having difficulty stitching it together. XUV stitch seemed appropriate since I can manually align the stacks, something I can't do in Metamorph, for example.
If anybody has came across a similar problem and knows the solution or has any suggestions, they will be greatly appreciated.
Thanks to all of you in advance,
Yevgeniy Romin
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