Brightness difference Hg vs LED

Posted by Oshel, Philip Eugene on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Brightness-difference-Hg-vs-LED-tp7581211.html

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All,

I had this question put to me by a new faculty member, and don't have a
ready answer:
"Is there a ballpark percentage for how much less bright an LED vs a
standard mercury lamp light?"
This is for regular epifluorescence, not confocal.

This is in the realm of arm-waving over a picture of beer (a good, dark
stout), ignoring brands, how old the Hg bulb is, ex/em cubes, which part
of the spectrum is used, and all that. Personally, I'd think the answer
is more like, "Doesn't matter, the dimmer system is still too bright to
use all the available light and not damage the specimen." But ... ?

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]