http://confocal-microscopy-list.275.s1.nabble.com/Brightness-difference-Hg-vs-LED-tp7581211p7581232.html
usually too much for our fluorochromes and can even damage plant cells. We
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>*****
>
>We have been testing a number of the solid state light sources. We do a
>lot
>of live cell imaging and they are all way too bright for live cells. We
>either add more ND filters so the cells don't die or we turn them way down
>in % output if that is an option on the light source. The companies seem
>to
>be making them brighter and brighter. I would love to know what
>applications
>people have that need all this light? We usually advise people to use less
>light and longer exposure times even with fixed samples.
>
>That being said we were able to get institutional support to replace our
>mercury sources as part of a sustainability program at McGill in a Mercury
>Free Microscopy initiative. This will save us lots of time and money in
>replacing the mercury bulbs all the time in addition to a reduction in
>mercury. Depending on how the sources are used they can also save a lot in
>power consumption too.
>
>We have been more interested in stability of the light sources on
>different
>time scales and they really perform well. Variability is in the single
>percentage range or less on most time scales. I would be happy to provide
>more information to anyone offline if they want to contact me.
>
>Sincerely,
>
>Claire