Re: 2P vs 1P psf

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-vs-1P-psf-tp7581269p7581294.html

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George,

thanks, I knew Anje Sporber's Paper but not the other one. Anyway, they
are good examples of what I meant: They show improved resolution but do
not give a FWHM formula a plain biologist can make use of. Again, if
somebody has an older reference with the formula for 3-photon
excitation, I would appreciate being pointed to it.

Steffen

On 18.11.2013 14:49, George McNamara wrote:

> *****
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>
>
> 3-photon confocal vs 3-photon PSF vs other modes (calculations) in
> figure 2 of Schrader, Bahlmann, Hell 1997 Optik
>
> http://www3.mpibpc.mpg.de/groups/hell/publications/pdf/Optik_104_116-124.pdf
>
>
> Here is a recent paper on tri-exciton (a 3 photon process) imaging (see
> references for earlier triexciton imaging):
> http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0064023

>
>
>
> enjoy,
>
> George
>
> On 11/18/2013 4:47 AM, Steffen Dietzel wrote:
>> *****
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>>
>> Citable literature is sparse in that area. For two-photon and confocal
>> resolution formula I usually cite these two, the first one being the
>> one that Guy already mentioned:
>>
>> G. Cox, C. J. Sheppard: Practical limits of resolution in confocal and
>> non-linear microscopy. In: Microscopy Research and Technique. Band 63,
>> Issue 1, 2004, p. 18–22, doi:10.1002/jemt.10423, PMID 14677129.
>>
>> B. Amos, G. McConnell, T. Wilson: Confocal microscopy. In: E. Egelman
>> (Ed.): Biophysical Techniques for Characterization of Cells (=
>> Comprehensive Biophysics. 2). Elsevier, Academic Press, Amsterdam
>> 2012, ISBN 978-0-12-374920-8, 2, p. 3–23,
>> doi:10.1016/B978-0-12-374920-8.00203-4
>> Author version:
>> http://www2.mrc-lmb.cam.ac.uk/images/groupleaders/Confocal_microscopy_Amos_McConnell_Wilson.pdf
>>
>>
>>
>> When it comes to three-photon excition it gets really thin. I just
>> very recently came accross a paper which gives the xy-resolution
>> formula (0.51*lambda)/(sqrt(3)*NA), see below. If anyone should have
>> an older citable reference. I very much would like to hear about it.
>>
>> Steffen
>>
>> Jung Ho Yu, Seung-Hae Kwon, Zdenˇek Petrášek3, Ok Kyu Park, Samuel
>> Woojoo Jun, Kwangsoo Shin, Moonkee Choi, Yong Il Park, Kyeongsoon
>> Park, Hyon Bin Na, Nohyun Lee, Dong Won Lee, Jeong Hyun Kim, Petra
>> Schwille, Taeghwan Hyeon: High-resolution three-photon biomedical
>> imaging using doped ZnS nanocrystals. In: Nature Materials. 12, 2013,
>> p. 359–366, doi:10.1038/NMAT3565
>>
>> On 17.11.2013 16:06, Jeff Reece wrote:
>>> *****
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>>>
>>> Is there a reference for the confocal psf being equal to the
>>> excitation psf when the pinhole=1AU (free access via the web is
>>> requested). It is not intuitive that the confocal psf would leave out
>>> the optics (pinhole) of the emission path.
>>>
>>> Thanks,
>>> Jeff
>>>
>>
>>
>
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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