Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/room-for-two-photon-microscopy-tp7581373p7581374.html
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My own experience with this very problem is that in terms of the room, it
is mainly the walls that matter. I have shiny aluminum shelves over the
`scope, and light grey vents and the like in the ceiling, and it doesn't
seem to make much difference. One key detail is that you want the walls
painted in matte black, not glossy. The contractors might mix this up so
be very specific. Now our biggest issue is the clothing worn by the
researchers; they were wearing white lab coats which was scattering light
from the computer monitors back towards the instruments. I have dark navy
blue lab coats on hand that solve that problem, or the researchers turn the
monitor off/away for long runs. If what you are doing is very picky (low
signal, lots of gain) a little bit of blackout cloth suspended in front of
the stage can help as well. We've (carefully) draped a bit over the
eyepieces on occasion, letting it hang down in front of the stage as a
quick but effective light shield.
Craig
On Mon, Dec 9, 2013 at 5:40 PM, Jurkevic, Aleksandr
<
[hidden email]>wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone: 573-882-4895
> Fax: 573-884-9676
>
>
>