http://confocal-microscopy-list.275.s1.nabble.com/room-for-two-photon-microscopy-tp7581373p7581379.html
Ok, but thinking outside the box, the key is to eliminate/control sources. As long as the NDD can’t see the sources you don’t need a black room. I would prefer a big box around the scope -it could be big enough to let you fiddle but still block the monitor which is a major source of light ion the room. Even a single shield between the monitor and scope will work and then use mat dark green/blue walls and use a red filter over the monitor giving a much more pleasant work environment.
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> I agree its best if you can just box in the microscope itself: Then you
> don't have to worry about the room so much. In our case, however, we work
> with a lot of live samples that require a lot of checking on, and plumbing
> and heaters that don't work well with an enclosure, which is why we
> darkened the entire room. That way you have relatively free access to poke
> and prod your sample. If you don't need easy access, or don't mind
> constantly opening the enclosure to check on your sample, the box method
> works very well and is certainly simpler. If you are building a room from
> scratch though and have the option, I still recommend painting the walls a
> dark colour as the cost difference will be small compared to the
> traditional white or beige paint and will always give an advantage.
>
> Craig
>
>
> On Mon, Dec 9, 2013 at 6:44 PM, Mark Cannell <
[hidden email]>wrote:
>
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>> There is no need to go so far as to paint walls black if you eliminate
>> light sources. A solid Faraday cage around the scope with a black curtain
>> and interior is just as good and fixes the monitor light pollution
>> problem. The room lighting should be easily dimmable from the
>> scope/workstation.
>>
>> HTH
>>
>> Mark
>>
>> On 10/12/2013, at 12:40 am, Jurkevic, Aleksandr <
[hidden email]>
>> wrote:
>>
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>>>
>>> Hello All,
>>>
>>> We are about to renovate the room for two-photon microscopy and I wonder
>> what suggestions you might have about reducing light pollution that affects
>> NDDs. As one of the measures, we are planning to paint walls and ceiling in
>> black. What about "small" things like aluminum electrical channels, wall
>> shelves, air vents? Have they also to be painted in dark colors or that
>> would be an overkill? I would appreciate if you share your solutions.
>>>
>>> Alexander
>>>
>>>
>>> Alexander Jurkevic, PhD
>>> Associate Director
>>> Molecular Cytology Core
>>> University of Missouri
>>> 120 Life Sciences Center
>>> 1201 E. Rollins St.
>>> Columbia, MO 65211-7310
>>>
>>> Phone: 573-882-4895
>>> Fax: 573-884-9676
>>>
>>>
>>
>> Mark B. Cannell Ph.D. FRSNZ
>> Professor of Cardiac Cell Biology
>> School of Physiology & Pharmacology
>> Medical Sciences Building
>> University of Bristol
>> Bristol
>> BS8 1TD UK
>>
>>
[hidden email]
>>
Mark B. Cannell Ph.D. FRSNZ