Re: room for two-photon microscopy

Posted by Wilson, Sean (LLU) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/room-for-two-photon-microscopy-tp7581373p7581380.html

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We used the K.I.S.S. principle on our ziess 710 NLO. Based on empirical evidence, we ended up draping blackout cloth across the top of the scope to keep out ambient light from the monitor, ect. & turned off external light sources (i.e. EXFO).

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Sean M. Wilson, Ph.D.
Assistant Professor, Center for Perinatal Biology
Technical Director, Advanced Imaging and Microscopy Core Facility
Loma Linda University School of Medicine Loma Linda, CA 92350
[hidden email]
909-558-4325 (Main Office)
x45209 (Note new campus extension)
x42183 (RW 1561 laboratory)
909-558-4029 (Fax)
909-647-7021 (Cell)

http://microscopy.llu.edu
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http://www.llu.edu/medicine/basic-sciences/faculty/pharmacology/wilson-sean.page?

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From: Confocal Microscopy List [[hidden email]] on behalf of Pascal Weber [[hidden email]]
Sent: Monday, December 09, 2013 6:27 PM
To: [hidden email]
Subject: Re: room for two-photon microscopy

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I work primarily on the mouse. I installed my microscope with a dark part and a
bright part (natural) to my surgeries. I had to create a black heated around my
upright microscope box. I have all the references to build oneself. I also modify
the plate to accept the sample. The walls were painted dark blue mat. But
anyway your laser should never leave the safety casing. Know that dark walls
absolutely no protection from parasites and dangerous reflections pulsed
infrared lasers. My Systems: Zeiss Maitai.
I'm building  a 2P + OPO. Coherent.