Re: room for two-photon microscopy
Posted by
Tobias Rose on
URL: http://confocal-microscopy-list.275.s1.nabble.com/room-for-two-photon-microscopy-tp7581373p7581385.html
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We usually use a dark cage as well. However, since we use monitors for visual stimulation of living animals we also synchronize the LED backlight of the screens to the turnaround phase of our resonant scanners. This way, the screens (both for stimulation and in some case also all remaining screens in the room) are only bright during the ~30% of scanning time where we reject the acquired data anyways. Works like a charm.
Tobias
(Leinweber et al. 2013, JOVE, in press)
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From: Confocal Microscopy List [mailto:
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Sent: Tuesday, December 10, 2013 01:40
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Subject: room for two-photon microscopy
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Hello All,
We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.
Alexander
Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310
Phone: 573-882-4895
Fax: 573-884-9676