Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multi-photon-Laser-tp7581421p7581422.html
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It is either a software calibration issue, which can be fixed by performing
a calibration procedure, or the AOM that modulates the laser isn't working
right. My guess would be that it is the former. Sometimes people forget
to save the calibration profile, or the calibration doesn't 'take' properly
into the software for some reason. If another attempt at calibration fails
then it might be an AOM hardware problem.
Craig Brideau
On Wed, Dec 18, 2013 at 2:15 PM, Claire Brown, Dr.
<
[hidden email]>wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
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> We recently had a service visit from Zeiss and they calibrated our
> multiphoton laser. It is not linear with relationship to the "slider" bar
> in ZEN. Has anyone had similar issues with their multi-photon system? It is
> a Zeiss LSM710 with a Coherent Ultra II laser.
>
> Sincerely,
>
> Claire
>