Re: room for two-photon microscopy: checking environment

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/room-for-two-photon-microscopy-tp7581373p7581424.html

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To add to the ventilation comment, we had a flexible tube installed near
the microscope connected to a filtered exhaust.  When we are using
isoflurane we can place the open end of the tube near the stage.  It has a
mild draw on it, not strong enough to disrupt the environment, but strong
enough to pull the gas away from the user.

Craig Brideau


On Thu, Dec 19, 2013 at 10:48 AM, Cammer, Michael <
[hidden email]> wrote:

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> If you are using Isoflurane or other gas based anesthesia, make sure the
> room is well ventilated even when you have a charcoal filter in the
> microscope's environmental chamber.
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU
> Langone Medical Center
> Cell:  914-309-3270   Lab: 212-263-3208
> http://ocs.med.nyu.edu/microscopy &
> http://www.med.nyu.edu/skirball-lab/dustinlab/
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Arvydas Matiukas
> Sent: Wednesday, December 11, 2013 5:03 PM
> To: [hidden email]
> Subject: Re: room for two-photon microscopy: checking environment
>
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> Hello List,
>
> We are going to move to new  research building with all current microscopy
> equipment. Few new microscopes ( confocal, 2-photon and
> STED-superresolution) will be installed as well.  I ordered all the walls
> painted matte black. I hope it makes a lot of difference . You already can
> notice that when making a photo.
> We installed red overhead light as an selectable option.
>
> Taking the occasion of the thread I would like to ask about other
> important issues to follow up before we move. Manufacturers of microscopes
> suggest to check the room for vibration, conditioning airflow, dust-free,
> clean power. Any feedback/advise is highly appreciated.
>
> Best regards,
> Arvydas
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> Department of Neurosci& Physiology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3167
> Syracuse, NY 13210
> tel.: [phone]
> fax: [phone]
> email: [hidden email]
> >>> "Jurkevic, Aleksandr" <[hidden email]> 12/9/2013 7:40 PM >>>
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> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>
>
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