Re: Multi-photon Laser

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multi-photon-Laser-tp7581421p7581431.html

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I would be a bit alarmed at 100's of mW of power coming out of the
objective (depending on the objective).  Normally one wants to scale the
power control so that if the user puts the slider to 100%, you get maximum
SAFE power out of the objective, rather than the maximum power the laser
will put out...

Craig


On Fri, Dec 20, 2013 at 2:26 AM, Andreas Bruckbauer <[hidden email]>wrote:

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> This surprises me, our service people always use the power meter, you need
> to make sure that you are in the linear range of the meter, as you can get
> several hundred mW of IR power out of the objective with todays lasers.
>
> -----Original Message-----
> From: "Claire Brown" <[hidden email]>
> Sent: 19/12/2013 21:57
> To: "[hidden email]" <[hidden email]>
> Subject: Re: Multi-photon Laser
>
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> Thanks for your input Craig. Here is a bit more detail about our situation.
>
> Our Zeiss service people tell us they do the calibration using the standard
> PMTs in the microscope which only measure up to 700 because of the optical
> separation on the 710 (non filter based). So they do the calibration at 699
> nm but we want it to be linear out into the IR. We are trying to see if
> there is an issue with their calibration technique as it doesn't seem
> ideal.
>
> That being said when we measure the laser output at 700 nm or 800 nm are
> not
> linear. This is after two service visits and calibrations when the service
> people tell us all is fine. So it is unclear if it is a calibration or AOM
> issue.
>
> Any input would be appreciated.
>
> Sincerely,
>
> Claire
>