Biological inert coverslip sealing

Posted by Antonio Jose Pereira on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biological-inert-coverslip-sealing-tp7581614.html

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Hi all,

For some purposes in live cell imaging we assemble a chamber with a coverslip on top of a slide.
We use two halves of another coverslip to act as spacers.

The  issue is the sealing. We've been using VALAP (a kind of wax) that we  melt and then use to seal all the way through the periphery of the  coverslip.

It works, but it would be more convenient to have something that does not have to be heated, and that would harden just by  contact with air.
A bit like the nail varnish used for fixed material ... but here we need something which is biologically inert.

Any suggestions? Thanks a lot.

Antonio



António Pereira
Chromosome Instability and Dynamics Group, Maiato lab

IBMC - Institute for Molecular and Cell Biology
Porto- Portugal


António Pereira
IBMC - Instituto de Biologia Molecular e Celular
Grupo Instabilidade e Dinâmica Cromossómica

Rua do Campo Alegre, 823
4150-180 Porto
Portugal

[phone] (ext# 1620)
[hidden email]