Posted by
Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biological-inert-coverslip-sealing-tp7581614p7581617.html
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Don't know if this will work for your purpose, but I used to make a ring (or square) with nail polish on the slide. let it dry and apply more layers to increase the thickness as needed. I let the nail polish dry out a couple of hours at room temperature (or faster in a 37oC oven) to evaporate solvents. This will make it life- and GFP-compatible. Put your specimen inside the well and add buffer/medium. Put a coverslip on top. The coverslip will typically stick to the nail polish, which serves both as a spacer and sealant.
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109
http://www.fhcrc.org/en.html--
On Feb 27, 2014, at 7:52 AM, Antonio J Pereira wrote:
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> Hi all,
>
> For some purposes in live cell imaging we assemble a chamber with a coverslip on top of a slide.
> We use two halves of another coverslip to act as spacers.
>
> The issue is the sealing. We've been using VALAP (a kind of wax) that we melt and then use to seal all the way through the periphery of the coverslip.
>
> It works, but it would be more convenient to have something that does not have to be heated, and that would harden just by contact with air.
> A bit like the nail varnish used for fixed material ... but here we need something which is biologically inert.
>
> Any suggestions? Thanks a lot.
>
> Antonio
>
>
>
> António Pereira
> Chromosome Instability and Dynamics Group, Maiato lab
>
> IBMC - Institute for Molecular and Cell Biology
> Porto- Portugal
>
>
> António Pereira
> IBMC - Instituto de Biologia Molecular e Celular
> Grupo Instabilidade e Dinâmica Cromossómica
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> Rua do Campo Alegre, 823
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